Application of Liquid Chromatography with Fluorimetric Detection for the Determination of Urinary Pentosidine in Type 2 Diabetes Mellitus Patients: Effect of Telmisartan Administration on Podocyturia and Relationship with Metabolic Control

Authors

  • Carlos Kornhauser UNIVERSITY OF GUANAJUATO
  • Gloria Barbosa-Sabanero UNIVERSITY OF GUANAJUATO
  • Noemí Gutierrez-Romero UNIVERSITY OF GUANAJUATO
  • Myrna Sabanero UNIVERSITY OF GUANAJUATO
  • Elva L. Perez-Luque UNIVERSITY OF GUANAJUATO
  • Armando Gómez-Ojeda UNIVERSITY OF GUANAJUATO

DOI:

https://doi.org/10.29356/jmcs.v62i2.390

Keywords:

Diabetic nephropathy, AGEs, Pentosidine, Telmisartan, Podocytes, Metabolic control

Abstract

The beneficial effects of a short period of Telmisartan administration were successfully assessed trough pentosidine urinary levels (uPen) and urinary podocyte excretion (UPE), in type 2 diabetes mellitus (DM2) patients. Patients with podocyturia received Telmisartan treatment (80 mg/day) for two months.  uPen were quantified pre and post treatment using HPLC with fluorimetric detection and in-lab synthesized standard. Immunofluorescence method for podocalyxin was used to evaluate urinary excretion of podocytes. uPen and UPE significantly decrease after treatment (p<0.01255 and p<0.005 respectively), as well as serum total cholesterol and LDL levels (p<0.001). These results suggest that podocyte protection by Telmisartan even in the face of deficient metabolic control could be an important matter in the prevention and progression of diabetic nephropathy. This study also strengthens evidence of the promising role of pentosidine as prognostic and diagnostic markers in diabetic nephropathy

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Author Biographies

Carlos Kornhauser, UNIVERSITY OF GUANAJUATO

MEDICAL SCIENCES DEPARTMENT

Gloria Barbosa-Sabanero, UNIVERSITY OF GUANAJUATO

MEDICAL SCIENCES DEPARTMENT

Noemí Gutierrez-Romero, UNIVERSITY OF GUANAJUATO

MEDICAL SCIENCES DEPARTMENT

Myrna Sabanero, UNIVERSITY OF GUANAJUATO

Department of Biology

Elva L. Perez-Luque, UNIVERSITY OF GUANAJUATO

MEDICAL SCIENCES DEPARTMENT

Armando Gómez-Ojeda, UNIVERSITY OF GUANAJUATO

MEDICAL SCIENCES DEPARTMENT

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Published

2018-06-06

Issue

Vol. 62 No. 2 (2018): Special Issue on “Modern Analytical Chemistry in Interdisciplinary Research”

Section

Regular Articles

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