Tetracycline Speciation Study in Aqueous Medium
DOI:
https://doi.org/10.29356/jmcs.v69i1.2293Keywords:
Tetracycline, stability, acidity constants, speciation, capillary zone electrophoresisAbstract
Tetracycline is a broad-spectrum antibiotic considered an emerging contaminant with major effects on human health and the ecosystems. Using the techniques of UV absorption spectroscopy and capillary zone electrophoresis, the characterization of tetracycline in aqueous medium is presented. Likewise, four acidity constants were determined: pKa1 = (3.277 ± 0.067), pKa2 = (7.397 ± 0.055), pKa3 = (9.349 ± 0.039) and pKa4 = (11.278 ± 0.024). Finally, capillary zone electrophoresis corroborates the predominance of five tetracycline species (TcH4+, TcH3, TcH2-, TcH2- and Tc3-) and presents a proposal for the deprotonation mechanism for tetracycline in aqueous medium.
Resumen. La tetraciclina es un antibiótico de amplio espectro, considerado como contaminante emergente con grandes afectaciones a la salud humana y el ecosistema. Mediante las técnicas de espectroscopía de absorción de UV y electroforesis capilar de zona se presenta la caracterización de la tetraciclina en medio acuoso. Así mismo se determinaron cuatro constantes de acidez: pKa1 = (3.277 ± 0.067), pKa2 = (7.397 ± 0.055), pKa3 = (9.349 ± 0.039) y pKa4 = (11.278 ± 0.024). Finalmente, a partir de electroforesis capilar de zona se corrobora el predominio de cinco especies de tetraciclina (TcH4+, TcH3, TcH2-, TcH2- y Tc3-) y se presenta una propuesta del mecanismo de desprotonación para tetraciclina en medio acuoso.
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Copyright (c) 2024 Luis Diego González-Garrido, Dafne Sarahia Guzmán-Hernández, Alberto Rojas-Hernández, Damaris Rodríguez-Barrientos, Jorge Juárez-Gómez, María Teresa Ramírez Silva
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