A Hydrogen Peroxide Biosensor Based on the Immobilization of the Highly Stable Royal Palm Tree Peroxidase (Roystonea regia) with Chitosan and Glutaraldehyde on Screen-printed Graphene Electrodes


  • Erika N. Villamizar Universidad Industrial de Santander
  • Carlos A. Ríos Universidad Industrial de Santander
  • John J. Castillo Universidad Industrial de Santander




Royal palm tree peroxidase, graphene, sensor, hydrogen peroxide.


This paper present the fabrication and electrochemical stud-ies of a screen-printed graphene electrode (SPGE) modified with Roy-al palm tree peroxidase (RPTP) in combination with chitosan (CS) and cross-linking of glutaraldehyde (GA) for detection of hydrogen perox-ide. Cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN)6]3-/4-) as a redox species demonstrated an increasing of 50mA evidenced by the electron transfer process of SPGE modified with CS-GA-RPTP. The graphene modified electrode exhibits excellent electrocatalytic activity to the reduction of H2O2, with a linear response in the 100 mM to 5 mM concentration range and a detection limit of 87 mM. The new sensor based on the modification of graphene electrode with the high stable RPTP will provide a potential amperometric detection system for determination of H2O2 in real samples with some biomedical or environmental importance.


Download data is not yet available.

Author Biographies

Erika N. Villamizar, Universidad Industrial de Santander

Grupo de Investigación en Bioquímica y Microbiología, GIBIM, Escuela de Química

Carlos A. Ríos, Universidad Industrial de Santander

Grupo de Investigación en Geología Básica y Aplicada, GIGBA, Escuela de Geología.

John J. Castillo, Universidad Industrial de Santander

Grupo de Investigación en Bioquímica y Microbiología, GIBIM, Escuela de Química


Chen, W.; Cai, S.; Ren, Q.; Wen, W.; Zhao, Y. Analyst. 2012, 137,


Lai, G.; Zhang, H.; Han, D. Microchim. Acta. 2009, 165, 159-165.

Li, M., Xu, S.; Tang, M. Electrochim. Acta. 2011, 56, 1144-1149.

Brusova, Z.; Ferapontova, E.; Sakharov, Y.; Magner, E. Electro-analysis. 2005, 17, 460-468.

Gaspar, S.; Catalin, I.; Gazaryan, I.; Bautista, G.; Sakharov, I.; Mattiason, B.; Csoregi, E. Electrochim. Acta. 2000, 46, 255-264.

Alpeeva, I.; Niculescu-nistor, M.; Castillo, J.; Csoregi, E.; Sakharov, I. Biosens. Bioelectron. 2005, 21, 742-748.

Sakharov, I. Biochem. 2004, 69, 1013-1020.

Zamorano, L.; Pina, D.; Arellano, J.; Bursakov, S.; Zhadan, A.; Calvete, J. Biochimie. 2008, 90, 1737-1749.

Walcarius, A.; Minteer, S.; Wang, J.; Lin, Y.; Mercoci, A. J. Mat. Chem. B. 2013, 1, 4878-4908.

Castillo, J.; Ferapontova, E.; Hushpulian, D. J. Electroanal. Chem. 2006, 588, 112-121.

Bin, X.; Chang, G.; Yi, X. Electrochim. Acta. 2012, 62, 327-334.

Niu, X.; Zhao, H.; Chen. C.; Lan, M. Electrochim. Acta. 2012, 65,


Matheus, P.; Abad, M.; Fernández, M. Rev. Tec. Ing. Univ. Zulia. 2007, 30, 225-235.

Sakharov, I.; Vesga, M.; Galaev, I.; Sakharova, I. Plant. Sci. 2001, 161, 853-860.

Shi, L.; Liu, X.; Niu, W. Biosen. Bioelectron. 2009, 24, 1159-1163.

Ahirwal, G.; Mitra, C. Sensors. 2009, 9, 881-894.

Chen, S.; Yuan, R.; Chai, Y. Electrochimica. Acta. 2009, 54, 3039-3046.

Farzana, S.; Ganesh, V.; Berchmans, S. J. Electrochm. Soc. 2013, 160, 573-580

Yagati, A.; Min, J.; Cho, S. J. Electrochem. Soc. 2014, 161, 133-140.

Wan, Q.; Song, H.; Shu, Z.; Wang, J.; Zou, N. (2013) Colloids. Surfaces. B. Biointerfaces. 2013, 104, 181-185.

Zhang, Q.; Qiao, Y.; Zhang, L.; Wu, S.; Zhou, H.; Xu, J.; Song, X. Electroanalysis. 2011, 23, 900-906.






Regular Articles