Methodology for Quantitative Determination of Polycyclic Aromatic Hydrocarbons in Protozoa Cultures
DOI:
https://doi.org/10.29356/jmcs.v55i2.841Keywords:
polycyclic aromatic hydrocarbons, matrix solid phase dispersion, solid phase extraction, high performance liquid chromatography- fluorescence, protozoan, Colpoda cucullus.Abstract
Two simple and sensitive analytical methods based on matrix solid phase dispersion (MSPD) and solid phase extraction (SPE), both followed by high performance liquid chromatography (HPLC) with fluorescence detection (FL) were developed for determining the high molecular weight polycyclic aromatic hydrocarbons (PAH) benzo(a)anthracene, benzo(b)fluoranthene and benzo(a)pyrene, in liquid cultures of the ciliate protozoan Colpoda cucullus. The first method employed MSPD technique for extraction of the analytes from a very small amount of microorganisms and insoluble organic matter.
The second method used SPE to extract analytes from the liquid medium. Validation parameters indicated good linearity (r2 > 0.99) andprecision (inter-day RSDs < 7%) for both methods. Recovery values were >90% for MSPD and 70% for SPE with limits of detection between 0.02 and 0.03 μg/g for MSPD-HPLC and between 0.03 and 0.04 μg/L for SPE-HPLC. The developed methodology was successfully used for the quantitative determination of PAH in microorganism cultures for the first time.
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Nalecz-Jawecki, G.; Demkowicz-Dobrzanski, K.; Sawicki, J. Sci. Total Environ. 1993, 134, 1227-1234.
Foissner, U. Agr. Ecosyst. Environ. 1997, 62, 93-103.
Fochtman, P.; Rasca, A.; Nierzedska, E. Environ. Toxicol. 2000, 15, 376-384.
Alekperon, I. Turk. J. Biol. 2000, 24, 309-320.
Berthold, A.; Jakl, T. JSS-J. Soil & Sediments 2002, 2, 170-190.
Belkin, S. Curr. Opin. Microbiol. 2003, 6, 206-212.
Schloter, I. M.; Dilly, O.; Munch, J. C. Agr. Ecosyst. Environ. 2003, 98, 255-262.
Gianfreda, L.; Rao, M. A. Crit. Rev. Env. Sci. Tec. 2008, 38, 269-310.
Torres, M. A.; Barros, M. P.; Campor, S. C. G. Pinto, E.; Rajamani, S.; Sayre, R.T.; Colepicolo, P. Ecotox. Environ. Safe. 2008, 71, 1-15.
Gutierrez, J. C.; Martín-González, A.; Díaz, S.; Ortega, R. Europ. J. Protistol. 2003, 39, 461-467.
Foissner, W. Agr. Ecosyst. Environ. 1999, 74, 95-112.
Campell, C. D.; Warren, A.; Cameron, C. M.; Hope, S. J. Chemosphere 1997, 34, 501-514.
Pratt, J. R.; Mochan, D.; Xu, Z. Bull. Environ. Contam. Toxicol. 1997, 58, 387-393.
Fernandez Leborans, G.; Olalla Herrero, Y.; Novillo, A. Ecotox. Environ. Safe. 1998, 39, 172-178.
Fernandez Leborans, G.; Olalla Herrero, Y.; Novillo, A. Ecotox. Environ. Safe. 2000, 47, 266-276.
Madoni, P. Environ. Pollut. 2009, 109, 53-59.
Rehman, A.; Shakoori, F. R.; Shakoori, A. R. Bull. Environ. Contam. Toxicol. 2006, 76, 907-913.
Rico, D.; Martín-Gonzalez, A.; Diaz, S.; De Lucas, P.; Gutierrez, J. C. Comp. Biochem. Phys. C. 2009, 149, 90-96.
Martín-González, A.; Díaz, S.; Borniquel, S.; Gallego, A.; Gutierrez, J. C. Res. Microbiol. 2006, 157, 108-118.
Díaz, S.; Martín-Gonzalez, A.; Gutiérrez, J. C. Environ. Int. 2006, 32, 711-717.
Guo, H-F.; Shi, Y- J.; Meng, F-Q.; Chen, J-S.; Lu, Y. L. Acta Ecologica Sinica 2006, 26, 70-74.
Mac Rae, I. C.; Vinckx, E. Soil Biol. Biochem. 1973, 5, 245-247.
Trielli, F.; Chessa, M. G.; Amaroli, A.; Ognibene, M.; Delmonte
Corrado, M. U. Chemosphere 2006, 65, 1731-1736.
Trielli, F.; Amaroli, A.; Sifredi, F.; Marchi, B.; Falugi, C.; Del Monte Corrado, M. Aquat. Toxicol. 2007, 83, 272-28.
Amaroli, F.; Trielli, F.; Sifredi, M.G.; Chessa, M. G.; Delmonte Corrado, M. Ecol. Indic. 2010, 10, 212-216
Asadullayena, E.; Alekperov, I. Tr. J. of Zoology 1999, 23, 275-289.
Lara, E.; Berney, C.; Ekelund, F.; Harms, H.; Chatzinotas, A. Soil Biology & Biochemistry 2007, 39, 139-148.
Lara, E.; Berney, C.; Harms, H.; Chatzinotas, A. FEMS Microb. Ecol. 2007, 62, 365-373.
Haritash, A. K.; Kaushik, C. P. J. Hazard. Mater. 2009, 169, 1-15.
Monteil-Rivera, F.; Betancourt, A.; Van Tra, H.; Yezza, A.; Hawari, J. J. Chromatogr. A 2007, 1154, 34-41.
Chen, Y-M.; Lee, T-H.; Lee, S-J.; Lin, J-Z.; Huang, R.; Chon, H-N. J. Chromatogr. B. 2006, 844, 134-141.
Cameán, A.; Moreno, I. M.; Ruiz, M. J.; Picó, Y. Anal. Bioanal. Chem. 2004, 380, 537-544.
Poster, D. L.; Schantz, M. M.; Sander, L. C.; Wise, S. A. Anal. Bioanal. Chem. 2006, 386, 859-881.
Andrade-Erroa, A.; Diévart, P.; Dagaut, P. Talanta 2010, 81, 265-274.
Filipkowska, A.; Lubecki, L.; Kowalewska, G. Anal. Chim. Acta 2005, 547, 243-254.
Khan, Z.; Troquet, J.; Vachelard, C. Int. J. Environ. Sci. Tech. 2005, 2, 275-286.
Titato, G. M.; Lanças, F. M. J Chromatogr Sci. 2006, 44, 35-40.
Barker, S. A. J. Biochem. Biophys. Methods 2007, 70, 151-62.
Díaz-Moroles, N. E.; Garza-Ulloa, H. J.; Castro-Ríos, R.; Ramírez-Villareal, E. G.; Barbarín-Castillo, J. M.; Salazar-Cavazos, M. L.; Waksman de Torres, N. J. Chromatogr. Sci. 2007, 45, 57-62.
Fidalgo-Used, N.; Blanco-González, E.; Sanz-Medel, A. Anal. Chim. Acta 2007, 590, 1-16.
Tang, B.; Isacsson, V. Energy & Fuels 2008, 22, 1425-1438.
Pena, M. T.; Casais, M. C.; Mejuto, M. C.; Cela, R. J. Chromatogr. A. 2010, 1217, 425-435.
Castro-Ortiz, L.P.; Luna-Pabello, V. M.; Villalobos Pietrini, R. Rev. Int. Contam. Ambient. 2007, 23, 35-45.
Marcé, R. M.; Borrul, F. J. Chromatogr. A 2000, 885, 273-289.
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