Immobilization of Thermomyces lanuginosus Lipase in PVA-alginate Beads
DOI:
https://doi.org/10.29356/jmcs.v55i3.817Keywords:
lipase, immobilized, PVA-alginate, Thermomyces lanuginosus.Abstract
Thermomyces lanuginosus lipase was immobilized in PVAalginate beads, obtaining immobilization % in the range of 94.4-98.4% using PVA concentrations ranging from 11% to 12.5%, and with cross linking times of 45 and 60 min using boric acid. Initial reactionrate was determined in free and immobilized state by hydrolysis of p-nitrophenol palmitate. Operational stability at different pH (4-7), agitation (100-500 r.p.m.), and temperature (40-80 °C) was investigated. Results showed that pH 6 and 7 no considerable loss of enzyme activity or enzyme was observed. At temperatures over 70 °C, enzyme suffers physical damage and showed a considerable loss of activity. No significant difference was observed when agitation was varied from 100 to 500 r.p.m.
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