Implementing Stepwise Solvent Elution in Multisyringe Liquid Chromatography (MSC) to Separate Norepinephrine, Epinephrine and Dopamine

Authors

  • María del Pilar Cañizares-Macías Universidad Nacional Autónoma de México
  • Hilda María González-San Miguel University of the Balearic Islands
  • Víctor Cerdà University of the Balearic Islands

DOI:

https://doi.org/10.29356/jmcs.v56i2.321

Keywords:

Multisyringe liquid chromatography, C18 monolithic column, catecholamines, stepwise elution

Abstract

A multisyringe liquid chromatographic (MSC) method by using stepwise solvent elution and reverse-phase liquid chromatography C18 monolithic column to separate Epinephrine (E), Norepinephrine (NE) and Dopamine (DA) was developed. The first step employs the mixture sodium1-hexanosulfonate monohydrate (HS) 7.5mM: methanol (98:2) to separate NE and E and the second step uses HS (5.0 mM): methanol (92:8) to elute DA. The three analytes were separated on 6 min spending only 10 mL of mobile phases. The detection limits were between 1.3 μg mL-1 (DA) and 2.04 μg mL-1 (NE).

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Author Biographies

María del Pilar Cañizares-Macías, Universidad Nacional Autónoma de México

Facultad de Química, Departamento de Química Analítica

Hilda María González-San Miguel, University of the Balearic Islands

Department of Chemistry

Víctor Cerdà, University of the Balearic Islands

Department of Chemistry

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Published

2017-10-12

Issue

Vol. 56 No. 2 (2012): Regular Issue

Section

Regular Articles

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