Study of the Influence of Microwave and Conventional Heating on the Lipase-Catalyzed Esterification of Lauric Acid with Different Alcohols

Authors

  • Velia Osuna Instituto Tecnológico de Tijuana
  • Ignacio A. Rivero 1. Instituto Tecnológico de Tijuana 2. Instituto Nacional de Investigaciones Nucleares

DOI:

https://doi.org/10.29356/jmcs.v56i2.318

Keywords:

Lipases, alcohols, esterification, microwave irradiation, lauric acid.

Abstract

In a systematic study, three commercial lipases were used for the esterification of lauric acid with six different alcohols: methanol, ethanol, propanol, butanol, pentanol, and allyl alcohol, under conventional heating and microwave irradiation. Optimization of reaction conditions included reaction time and effect of temperature under microwave irradiation and conventional heating. The optimum reaction temperature was previously established in enzymatic esterification by conventional heating. Novozyme 435, lipase (Pseudomonas cepacia) immobilized in diatomite (PS-DI) and lipase (P. cepacia) immobilized in ceramic (PS-CI) were used in this study. The latter showed the highest activity, therefore, this enzyme was used to investigate the variation of reaction time under microwave heating. The stability of immobilized lipases under classical heating and under the microwave irradiation was studied by visual analysis of the immobilized matrix by scanning electron microscopy (SEM).

Downloads

Download data is not yet available.

Author Biographies

Velia Osuna, Instituto Tecnológico de Tijuana

Centro de Graduados e Investigación

Ignacio A. Rivero, 1. Instituto Tecnológico de Tijuana 2. Instituto Nacional de Investigaciones Nucleares

  1. Centro de Graduados e Investigación
  2. Departamento de Química

Downloads

Published

2017-10-12

Issue

Section

Regular Articles