Identification of Anthocyanins in Red Grape, Plum and Capulin by MALDI-ToF MS

Authors

  • Araceli Castañeda-Ovando Universidad Autónoma del Estado de Hidalgo
  • Ondra Sedo Masaryk University
  • Josef Havel Masaryk University
  • Lourdes Pacheco Universidad Autónoma del Estado de Hidalgo
  • Carlos Andrés Galán-Vidal Universidad Autónoma del Estado de Hidalgo
  • Elizabeth Contreras López Universidad Autónoma del Estado de Hidalgo

DOI:

https://doi.org/10.29356/jmcs.v56i4.247

Keywords:

MALDI-ToF MS, Grapes, Plums, Capulins, Deprotonated Anthocyanins

Abstract

A simple and fast method was developed to identify anthocyanins in red grapes, plums and capulins by MALDI-ToF MS. Three different matrices were used: α-CHCA (α-cyano-4-hydroxycinnamic acid), THA (2’,4’,6’-trihydroxyacetophenone) and DHBA (2,5-dihydroxybenzoic acid). Post-Source Decay (PSD) fragment detection allowed studying the structure of compounds detected. Deprotonated anthocyanin species were assigned in capulin ([Cyanidin-3-glucosidedeprotonated] K+ and [Cyanidin-3-rutinoside-deprotonated]K+), due to these compounds also can be ionized in form of potassium adducts combined with a deprotonation.

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Author Biographies

Araceli Castañeda-Ovando, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

Ondra Sedo, Masaryk University

Analytical Chemistry Department

Josef Havel, Masaryk University

Analytical Chemistry Department

Lourdes Pacheco, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

Carlos Andrés Galán-Vidal, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

Elizabeth Contreras López, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

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Published

2017-10-12

Issue

Section

Regular Articles

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