Antimicrobial and Antioxidant Activities of Four Essential Oils
DOI:
https://doi.org/10.29356/jmcs.v68i4.2309Keywords:
Essential oils, composition, antimicrobial activity, antioxidant capacityAbstract
Various opportunistic microorganisms, such as bacteria and fungi, are responsible for multiple infectious diseases, which represent a threat to global health. Essential oils (EOs) have shown antimicrobial and antioxidant properties, making them an excellent alternative to control multi-resistant bacteria. In this work, for the first time, the antimicrobial and antioxidant activities of four EOs were evaluated, namely Trixis angustifolia DC (EOTA), Dalea bicolor Humb & Bonpl. Ex Willd (EODB), Tagetes parryi A.Gray (EOTP) and Eupatorium glabratum Kunth (EOEG). They were obtained by hydrodistillation, and their chemical composition was determined by GC-MS (Gas chromatography-mass spectroscopy) using HP5-MS column. Their antimicrobial and antioxidant activities were determined by the microdilution method and the DPPH and ABTS techniques, respectively. The main compounds of the EOs were piperitone (36.67 %) for EOTA, β-pinene (27.25) for EODB, verbenone (31.13 %) for EOTP and α-cadinol (7.78 %) and bornyl acetate (6.45 %) for EOEG. The EOs EOTA, EODB, EOTP and EOEG inhibited the development of Candida at a concentration of 62.5–500 µg/mL, whereas the antibacterial activities of these oils were observed at concentrations from 125–500 µg/mL. The antioxidant activity of EOTA and EODB were IC50 = 0.641, 1.195 mg/mL, whereas those of EOTP and EOEG was lower. These results show that four EOs have antimicrobial activity.
Resumen. Diversos microorganismos oportunistas, como bacterias y hongos, son responsables de múltiples enfermedades infecciosas, que representan una amenaza para la salud mundial. Los aceites esenciales (EOs) han demostrado propiedades antimicrobianas y antioxidantes, lo que los convierte en una excelente alternativa para el control de bacterias multirresistentes. En este trabajo, por primera vez, se evaluaron las actividades antimicrobianas y antioxidantes de cuatro EOs: Trixis angustifolia DC (EOTA), Dalea bicolor Humb & Bonpl. Ex Willd (EODB), Tagetes parryi A.Gray (EOTP) y Eupatorium glabratum Kunth (EOEG). Los aceites se obtuvieron por hidrodestilación y se determinó su composición química por GC-MS (cromatografía de gases-espectrometría de masas) utilizando una columna HP5-MS. Sus actividades antimicrobiana y antioxidante se determinaron por el método de microdilución y las técnicas DPPH y ABTS, respectivamente. Los principales compuestos de los aceites esenciales fueron piperitona (36,67 %) para EOTA, β-pineno (27,25 %) para EODB, verbenona (31,13 %) para EOTP y α-cadinol (7,78 %) y acetato de bornilo (6,45 %) para EOEG. Los aceites esenciales EOTA, EODB, EOTP y EOEG inhibieron el desarrollo de Candida a una concentración de 62,5–500 μg/mL, mientras que las actividades antibacterianas de estos aceites se determinaron a concentraciones de 125–500 μg/mL. La actividad antioxidante de EOTA y EODB fue de IC50 = 0,641, y 1,195 mg/mL respectivamente, mientras que las de EOTP y EOEG fueron menores. Estos resultados muestran que los cuatro EOs tienen actividad antimicrobiana.
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Arbune, M.; Gurau, G.; Niculet, E.; Iancu, A. V.; Lupasteanu, G.; Fotea, S.; Vasile, M. C.; Tatu, A. L. Infect Drug Resist. 2021, 14, 2369–2378. DOI: https://doi.org/10.2147/IDR.S312231.
De Oliveira, D. M. P.; Forde, B. M.; Kidd, T. J.; Harris, P. N. A.; Schembri, M. A.; Beatson, S. A.; Paterson, D. L.; Walker, M. Clin Microbiol. Rev. 2020, 33, 1-49. DOI: https://doi.org/10.1128/cmr.00181-19.
Balaban, N. Q.; Helaine, S.; Lewis, K.; Ackermann, M.; Aldridge, B.; Andersson, D. I.; Brynildsen, M. P.; Bumann, D.; Camilli, A.; Collins, J. J.; Dehio, C.; Fortune, S.; Ghigo, J. M.; Hardt, W. D.; Harms, A.; Heinemann, M.; Hung, D. T.; Jenal, U.; Levin, B. R.; Michiels, J.; Zinkernagel, A. Nat. Rev. Microbiol. 2019, 17, 441–448. DOI: https://doi.org/10.1038/s41579-019-0196-3.
Silva, S.; Negri, M.; Henriques, M.; Oliveira, R.; Williams, D. W.; Azeredo, J. FEMS Microbiol. Rev. 2012, 36, 288–305. DOI: https://doi.org/10.1111/j.1574-6976.2011.00278.x.
Soulaimani, B.; Varoni, E.; Iriti, M.; Mezrioui, N. E.; Hassani, L.; Abbad, A. Antibiotics. 2021, 10, 1049. DOI: https://doi.org/10.3390/antibiotics10091049.
Köhler, J. R.; Hube, B.; Puccia, R.; Casadevall, A.; Perfect, J. R. Microbiol. Spectr. 2017, 5. DOI: https://doi.org/10.1128/microbiolspec.FUNK-0014-2016.
Pristov, K. E.; Ghannoum, M. A. Clin Microbiol. Infect. 2019, 25, 792–798. DOI: https://doi.org/10.1016/j.cmi.2019.03.028.
Paur, I.; Carlsen, M. H.; Halvorsen, B. L.; Blomhoff, R. in: Herbal Medicine: Biomolecular and Clinical Aspects. Chapter 2. Benzie, I. F., Wachtel, G. S., Ed., CRC Press/Taylor&Francis, Boca Raton, (FL), 2011, 358.
Bakkali, F.; Averbeck, S.; Averbeck, D.; Idaomar, M. FCT. 2008, 46, 446-475. DOI: https://doi.org/10.1016/j.fct.2007.09.106.
Croteau, R. J. Herbs. Spices Med. Plants. 1986, 1, 81-135.
Gerchenzon, J.; Dudareva, N. Nat. Chem. Biol. 2007, 3, 408-414. DOI: https://doi.org/10.1038/nchembio.2007.
Bilia, A.R.; Guccione, C.; Isacchi, B.; Righeschi, C.; Firenzuoli, F.; Bergonzi, M.C. Evid Based Complement Alternat Med. 2014, 2014., 51593. DOI: https://doi.org/10.1155/2014/651593.
Najar, B.; Shortrede, J. E.; Pistelli, L.; Buhagiar, J. Chem, Biodivers. 2020, 17, e1900478. DOI: https://doi.org/10.1002/cbdv.201900478.
São Pedro, A.; Santo, I.; Silva, C.; Detoni, C.; Albuquerque, E. in: Microbial pathogens and strategies for combating them; Vol. 4, Méndez-Vilas, A., Ed.; Formatex Res. Center Pulisher, Badajoz, 2013, 1364-1374.
Fan, M.; Zhang, X.; Song, H.; Zhang, Y. Molecules. 2023, 28, 5022. DOI: https://doi.org/10.3390/molecules28135022.
Sharifi-Rad, J.; Soufi, L.; Ayatollahi, S. A.; Iriti, M.; Sharifi-Rad, M.; Varoni, E. M.; Shahri, F.; Esposito, S.; Kuhestani, K.; Sharifi-Rad, M. Cell Mol. Biol. (Noisy-le-grand). 2016, 62, 69-74. DOI: https://doi.org/10.14715/cmb/2016.62.9.11.
Candan, F.; Unlu, M.; Tepe, B.; Daferera, D.; Polissiou, M.; Sökmen, A.; Akpulat, H.A. J Etnopharmacol. 2003, 87, 215-220. DOI: https://doi.org/10.1016/s0378-8741(03)00149-1.
Al-Hajj, N.Q.; Wang, H.X.; Ma, C.; Lou, Z.; Bashari, M.; Thabit, R. Trop. J. Pharm. Res. 2014, 13, 1287-1293. DOI: https://doi.org/10.4314/tjpr.v13i8.13.
De Santi, I.I.; Gatto, D.A.; Machado, M.R.G.; Dos Santos, P.S.B.; Freitag, R.A. Am. J. Plant Sci. 2017, 8, 1560-1571. DOI: https://doi.org/10.4236/ajps.2017.87108.
Sánchez-Chávez, A. C., Salazar-Gómez, A., Zepeda-Vallejo, L. G., Hernández de Jesús, M. L., Quintos-Escalante, M.; Vargas-Díaz, M. E.; Luna-Herrera, J. Nat. Prod. Res. 2019, 33, 1477–1481. DOI: https://doi.org/10.1080/14786419.2017.1416381.
García-Sánchez, E.; Ramírez-López, C. B.; Martínez-Muñoz, R. E.; Flores-García, A.; Río, R. E. D.; Martínez-Pacheco, M. M. Polibotánica. 2015, 39, 91-101.
Morales-Ubaldo, YA; Rivero-Perez, N.; Morales-Ubaldo, AL; Valladares-Carranza, B.; López-Rodríguez, GM; Zaragoza-Bastida, A. J. Vet. Res. 2022, 33, 1-6.
González-Velasco, H. E.; Pérez-Gutiérrez, M. S.; Alonso-Castro, Á. J.; Zapata-Morales, J. R.; Niño-Moreno, P. D. C.; Campos-Xolalpa, N.; González-Chávez, M. M. Molecules. 2022, 27, 2612-2620. DOI: https://doi.org/10.3390/molecules27092612.
National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that Grow Aerobically; Approved Standard—Tenth Edition NCCLS: Villanova, PA, USA, 2015.
CLSI. Reference method for broth dilution antifungal susceptibility testing of yeast; fourth informational supplement. CLSI document 341 M27-S4. Wayne, PA: Clinical and Laboratory Standards Institute; 2012.
Cascaes, M. M.; De Moraes, Â. A. B.; Cruz, J. N.; Franco, C. J. P.; E Silva, R. C.; Nascimento, L. D. D.; Ferreira, O. O.; Anjos, T. O. D.; de Oliveira, M. S.; Guilhon, G. M. S. P.; Andrade, E. H. A. Antioxidants. 2022, 11, 1709. DOI: https://doi.org/10.3390/antiox11091709.
Thaipong, B.; Unaroj, K.; Crosby, L.; Cisneros-Zevallos, L.; Hawkins D.B. J. Food Compost. Anal. 2006, 19, 669-675. DOI: https://doi.org/10.1016/j.jfca.2006.01.003.
Esmaeili, A.; Moaf, L.; Rezazadeh, S. J. Essent. Oil Bear Pl. 2014, 17, 664-669.
Dadgostar, P. Infec. Drug Resist. 2019, 12, 3903–3910. DOI: https://doi.org/10.2147/IDR.S234610.
Ajetunmobi, O. H.; Badali, H.; Romo, J. A.; Ramage, G.; Lopez-Ribot, J. L. Biofilm. 2023, 5, 100126. DOI: https://doi.org/10.1016/j.bioflm.2023.100126.
Iraji, A.; Yazdanpanah, S.; Alizadeh, F.; Mirzamohammadi, S.; Ghasemi, Y.; Pakshir, K.; Yang, Y.; Zomorodian, K. J. Applied Microbiol. 2020, 129, 1541–1551. DOI: https://doi.org/10.1111/jam.14740.
Ivanov M.; Kannan A.; Stojković D.S.; Glamočlija J.; Calhelha R.C.; Ferreira I.C.F.R.; Sanglard D.; Soković M. Int. J. Mol. Sci. 2021, 6, 483-499. DOI: https://doi.org/10.3390/ijms22020483.
Zengin, H.; Baysal, A.H. Molecules. 2014, 19, 17773-7798. DOI: https://doi.org/10.3390/molecules191117773.
Shirazi, M.T.; Gholami, H.; Kavoosi, G.; Rowshan, V.; Tafsiry, A. Food Sci. Nutr. 2014, 2, 146–155. DOI: https://doi.org/10.1002/fsn3.85.
Rivas da Silva, A.C.; Lopes, P.M.; Barros de Azevedo, M.M.; Costa, D.C.; Alviano, C.S.; Alviano, D.S. Molecules. 2012, 17, 6305-6316. DOI: https://doi.org/10.3390/molecules17066305.
El Moussaoui, A.; Bourhia, M.; Jawhari, F.Z.; Salamatullah, A.M.; Ullah, R.; Bari, A.; Majid Mahmood, H.; Sohaib, M.; Serhii, B.; Rozhenko, A.; Aboul-Soud, M.A.M.; Ezzeldin, E.; Mostafa, G.A.E.; Bousta, D.; Bari, L. Appl. Sci. 2021, 11, 5168. DOI: https://doi.org/10.3390/app11115168.
Orchard, A.; van Vuuren, S. Alternat. Med. 2017, 2017, 4517971. DOI: https://doi.org/10.1155/2017/4517971.
Chebbac, K.; Ghneim, H.K.; El Moussaoui, A.; Bourhia, M.; El Barnossi, A.; Benziane Ouaritini, Z.; Salamatullah, A.M.; Alzahrani, A.; Aboul-Soud, M.A.; Giesy, J.P; Guemmouh, R. Molecules. 2022, 27, 1136. DOI: https://doi.org/10.3390/molecules27031136.
Esmaeili, A.; Panahi, Z. A.; Ebrahimzadeh, M. A. J. Essen. Oil Bear Plants. 2014, 17, 806-812. DOI: https://doi.org/10.1080/0972060X.2014.895203.
Peyrusson, F.; Nguyen, T.K.; Najdovski, T.; Van Bambeke, F. Microbiol. Spectr. 2022, 10, e02313-21. DOI: https://doi.org/10.1128/spectrum.02313-21.
Kim, D.O.; Lee, K.W.; Lee, H.J.; Lee, C.Y. J. Agric. Food Chem. 2002, 50, 3713–3717. DOI: https://doi.org/10.1021/jf020071c.
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Copyright (c) 2024 Salud Pérez-Gutiérrez, Ana Laura Esquivel-Campos, Leonor Sánchez Pérez, Marco González Martínez, Aranxa Reyes Ponce, Julia Pérez Ramos, Ernesto Zapata Flores
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