Phytochemical profile, antioxidant properties and protein contents of Astragalus tenuifoliosus seeds

Authors

  • Maher Mahmoudi Arid Region Institute https://orcid.org/0000-0002-4114-2959
  • Fayçal Boughalleb University of Tunis El-Manar
  • Mahmoud Mabrouk University of Gabes
  • Raoudha Abdellaoui University of Gabes

DOI:

https://doi.org/10.29356/jmcs.v67i1.1854

Keywords:

Astragalus tenuifoliosus seeds, proteins, globulins, quinic acid, LC-ESI/MS.

Abstract

Fabaceae seeds are reported to be used for varying medicinal and pharmaceutical purposes. However, knowledge of the nutritive value of Astragalus tenuifoliosus seeds is largely based on very limited data and remains unexplored, we report here the protein content, phenolics as well as the antioxidant potential of plant seeds to give adequate information on its suitability as a new source of natural bioactive compounds. The protein content was determined using the Kjeldahl and Bradford assays. The phytochemical contents were evaluated, and the extracts were further subjected to high-performance liquid chromatography-electrospray ionization – mass spectrometry (HPLC-ESI-MS) analysis. The antioxidant potential was evaluated using the total antioxidant capacity and the free DPPH radical scavenging activity. The results obtained from the protein analysis showed that the total content was 59.43 % of the dry matter basis. The globulins constituted the dominant fraction and followed by albumins, glutelins, and prolamins. The phytochemical investigation showed considerable amounts of polyphenol, flavonoid and condensed tannin amounts. The LC-ESI/MS analysis revealed the presence of 18 phenolics including 8 phenolic acids and 10 flavonoids mostly predominated by quinic acid (255.4 µg g-1 DW), p-coumaric acid (65.39 µg g-1 DW), quercetin (97.21 µg g-1 DW), and cirsiliol (29 µg g-1 DW). The seeds possessed strong antioxidant potential evidenced by their DPPH radical scavenging activities and total antioxidant capacity. The obtained findings contribute to the limited bibliographic information concerning A. tenuifoliosus seeds and represent a starting point to evaluate its potential as a valuable source of proteins, natural antioxidants, and safe bioactive compounds.

 

Resumen. Las semillas de las Fabaceae se utilizan para diversos fines medicinales y farmacéuticos. Sin embargo, el conocimiento del valor nutritivo de las semillas de Astragalus tenuifoliosus se basa en gran medida en datos muy limitados y sigue sin explorarse. Aquí se reporta el contenido de proteínas, fenoles y el potencial antioxidante de las semillas de plantas para brindar información adecuada sobre su idoneidad como nueva fuente de compuestos bioactivos naturales. El contenido de proteína se determinó utilizando los ensayos de Kjeldahl y Bradford. Se evaluaron los contenidos fitoquímicos y los extractos se sometieron a análisis de cromatografía líquida de alto rendimiento, ionización por electropulverización y espectrometría de masas (HPLC-ESI-MS). El potencial antioxidante se evaluó utilizando la capacidad antioxidante total y la actividad de captación de radicales libres DPPH. Los resultados obtenidos del análisis de proteína mostraron que el contenido total fue de 59.43% con base en el peso seco. Las globulinas constituyeron la fracción dominante, seguidas por las albúminas, las glutelinas y las prolaminas. La investigación fitoquímica mostró cantidades considerables de polifenoles, flavonoides y taninos condensados. El análisis medinate LC-ESI/MS reveló la presencia de 18 fenoles, incluidos 7 ácidos fenólicos y 11 flavonoides, predominando en su mayoría el ácido quínico (255.4 µg g-1 DW), el ácido p-cumárico (65.39 µg g-1 DW), la quercetina (97.21 µg g-1 DW), y el cirsiliol (29 µg g-1 DW). Las semillas poseen un fuerte potencial antioxidante, evidenciado por sus actividades de eliminación de radicales DPPH y su capacidad antioxidante total. Los hallazgos obtenidos contribuyen a la limitada información bibliográfica sobre las semillas de A. tenuifoliosus y representan un punto de partida para evaluar su potencial como fuente valiosa de proteínas, antioxidantes naturales y compuestos bioactivos seguros.

Downloads

Download data is not yet available.

Author Biographies

Fayçal Boughalleb, University of Tunis El-Manar

Laboratory of Plant, Soil and Environment Interactions (LR21ES01), Faculty of Sciences of Tunis

Mahmoud Mabrouk, University of Gabes

Platform Advances Analysis, Institute of Arid Regions

Raoudha Abdellaoui, University of Gabes

Platform Advances Analysis, Institute of Arid Regions

References

Maassoumi, A. A. Astragalus in the Old World: check-list. Research Institute of Forests and Rangelands, 1998, 1-617.

Podlech, D.; Zarre, S. in: Leguminosae in the Old World. Vol.2, 2013, 2, 1039-1150.

Lock, J. M.; Simpson, K. Royal Botanic Gardens, Kew, 1991.

Li-Man, S.; Xiao-Ling, W.; Wen-Long, D.; Li-Sheng, D.; Shu-Lin, P. Chin. J. Nat. Med. 2011, 9, 38-41. DOI: https://doi.org/10.1016/S1875-5364(11)60017-4+

Niu, Y.; Wang, H.; Xie, Z.; Whent, M.; Gao, X.; Zhang, X.; Zou, S.; Yao, W.; Yu, L. Food Chem. 2011, 128, 620-626. DOI: https://doi.org/10.1016/j.foodchem.2011.03.055+

Golmohammadi, F. Tech. J. Eng. Appl. Sc. 2013, 3, 3702-3721.

Khyade, M.; Kamble, S.; Waman, M.; Padwal, A.; Gunjal, M. Curr. Nutr. Food Sci. 2020, 16, 1381-1392. DOI: https://doi.org/10.2174/1573401316666200221110140+

Cardozo, M. L.; Ordoñez, R. M.; Zampini, I. C.; Cuello, A. S.; Dibenedetto, G.; Isla, M. I. Food Rese. Int. 2010, 43, 1505-1510. DOI https://doi.org/10.1016/j.foodres.2010.04.004+

Mahmoudi, M.; Abdellaoui, R.; Boughalleb, F.; Yahia, B.; Mabrouk, M.; Nasri, N. Food Chem. 2021, 339, 127824. DOI: https://doi.org/10.1016/j.foodchem.2020.127824

Rios, J.; Waterman, P. Phytother. Res. 1997, 11, 411-418. DOI: https://doi.org/10.1002/(SICI)1099-1573(199709)11:6<411::AID-PTR132>3.0.CO;2-6

Ozenda, P. Flora of the Sahara. CNRS, Ed. Paris 1977.

Greuter, W.; Raus, T. Willdenowia. 1989, 27-48.

Mahdhi, M.; Houidheg, N.; Mahmoudi, N.; Msaadek, A.; Rejili, M.; Mars, M. Pol. J. of Microbiol. 2016, 65, 331-339.

Day, L. Trends Food Sci. Technol. 2013, 32, 25-42. DIO: https://doi.org/10.1016/j.tifs.2013.05.005

Mahmoudi, M.; Abdellaoui, R.; Boughalleb, F.; Yahia, B.; Bouhamda, T.; Bakhshandeh, E.; Nasri, N. Ind. Crops Prod. 2020, 151, 112462. DOI: https://doi.org/10.1016/j.indcrop.2020.112462

Boughalleb, F.; Mahmoudi, M.; Abdellaoui, R.; Yahia, B.; Zaidi, S.; Nasri, N. J. Food Biochem. 2020, 44(1), e13093. DOI: https://doi.org/10.1111/jfbc.13093

Al-Qassabi, J. S. A.; Weli, A. M.; Hossain, M. A. Sustainable Chem. Pharm. 2018, 8, 71-75. DOI: https://doi.org/10.1016/j.scp.2018.03.001

Kohen, R.; Nyska, A. Toxicol. Pathol. 2002, 30, 620-650. DOI: https://doi.org/10.1080/019262302901667

Knekt, P.; Jarvinen, R.; Reunanen, A.; Maatela, J. Bmj. 1996, 312, 478-481. DOI : https://doi.org/10.1136/bmj.312.7029.478

20. Mozafari, M. R.; Flanagan, J.; Matia‐Merino, L.; Awati, A.; Omri, A.; Suntres, Z. E.; Singh, H. J. Sci. Food Agric. 2006, 86, 2038-2045. DOI: https://doi.org/10.1002/jsfa.2576

Luximon-Ramma, A.; Bahorun, T.; Soobrattee, M. A.; Aruoma, O. I. J. Agric. Food Chem. 2002, 50, 5042-5047. DOI: https://doi.org/10.1021/jf0201172

AOAC. Official Methods of Analysis (16th edn). AOAC International, Arlington, VA (1995)., 1995.

Ortiz, J.; Romero, N.; Robert, P.; Araya, J.; Lopez-Hernández, J.; Bozzo, C.; Navarrete, E.; Osorio, A.; Rios, A. Food chem. 2006, 99, 98-104. DOI: https://doi.org/10.1016/j.foodchem.2005.07.027

Dewanto, V.; Wu, X.; Adom, K. K.; Liu, R. H. J. Agric. Food Chem. 2002, 50, 3010-3014. DOI: https://doi.org/10.1021/jf0115589

Helrich, K. 1990, Official methods of analysis of the Association of Official Analytical Chemisty. AOAC, Ed. Arlington, 1990.

Broadhurst, R. B.; Jones, W. T. J. Sci. Food Agric. 1978, 29, 788-794. DOI: https://doi.org/10.1002/jsfa.2740290908

Mahmoudi, M.; Boughalleb, F.; Maaloul, S.; Zaidi, S.; Bakhshandeh, E.; Abdellaoui, R. Sci. Hortic. 2023, 309, 111616. DOI : https://doi.org/10.1016/j.scienta.2022.111616

Sánchez‐Moreno, C.; Larrauri, J. A.; Saura‐Calixto, F. J. Sci. Food Agric. 1998, 76, 270-276. DOI: https://doi.org/10.1002/(SICI)1097-0010(199802)76:2%3C270::AID-JSFA945%3E3.0.CO;2-9

El Naga, A.; Abou Rizk, R., in: Proceeding 1st Egyptian & Syrian Conference for Agriculture & Food, El Minia., 1:143, 2003.

Smith Jr, C.; Earle, F.; Wolff, I.; Jones, Q. J. Agric. Food Chem. 1959, 7, 133-136

Van Etten, C. H.; Kwolek, W. F.; Peters, J. E.; Barclay, A. S. J. Agric. Food Chem. 1967, 15, 1077-1089.

Bakoğlu, A.; Bağcı, E.; Ciftci, H. J. Food Agric. Environ. 2009, 7, 343-346.

Heger, J.; Eggum, B. J. Cereal Sci. 1991, 14, 63-71. DOI: https://doi.org/10.1016/S0733-5210(09)80018-0

Boughalleb, F.; Mahmoudi, M.; Abdellaoui, R.; Yahia, B.; Zaidi, S.; Nasri, N. J. Food biochem. 2020, e13093. DOI: https://doi.org/10.1111/jfbc.13093

Alamanou, S.; Doxastakis, G. Food Hydrocolloids. 1997, 11, 409-413. DOI: https://doi.org/10.1016/S0268-005X(97)80038-0

Niknam, V.; Ebrahimzadeh, H. Pak. J. Bot. 2002, 34, 283-289

Žilić, S.; Šukalović, V.; Maksimović, V.; Maksimović, M.; Basić, Z.; Perić, V.; Maksimović, J. D., in: Proceedings 46th Croatian and 6th International Symposium on Agriculture. Opatija, Croatia, Vol. 689, 2005.

Chen, R.Z.; Tan, L.; Jin, C.G.; Lu, J.; Tian, L.; Chang, Q.Q.; Wang, K. Ind. Crops Prod. 2015, 77, 434-443. DOI: https://doi.org/10.1016/j.indcrop.2015.08.006

Labed, A.; Ferhat, M.; Labed-Zouad, I.; Kaplaner, E.; Zerizer, S.; Voutquenne-Nazabadioko, L.; Alabdul Magid, A.; Semra, Z.; Kabouche, A.; Kabouche, Z. Pharmaceutical biology. 2016, 54, 3026-3032. DOI: https://doi.org/10.1080/13880209.2016.1200632

Teyeb, H.; Zanina, N.; Neffati, M.; Douki, W.; Najjar, M. F. Turk. J. Biol. 2012, 36, 53-58 DOI: https://doi.org/10.3906/biy-1010-131

Pistelli, L., in: Studies in natural products chemistry, Vol. 27, Elsevier, 2002, 443-545.

Pero, R. W.; Lund, H. International Journal of Biotechnology and Biochemistry. 2009, 5, 293-305

Draths, K.; Knop, D. R.; Frost, J. J. Am. Chem. Soc. 1999, 121, 1603-1604. DOI: https://doi.org/10.1021/ja9830243

Downloads

Published

2023-01-01

Issue

Section

Regular Articles