Amino Acid Sequence Determination and Chemical Synthesis of CllErg1 (γ-KTx1.5), a K+ Channel Blocker Peptide Isolated from the Scorpion Centruroides limpidus limpidus

Authors

  • Fredy I. Corona Universidad Nacional Autónoma de México
  • Cipriano Balderas Universidad Nacional Autónoma de México
  • Liliana Pardo-López Universidad Nacional Autónoma de México
  • Lourival D. Possani Universidad Nacional Autónoma de México
  • Georgina B. Gurrola Universidad Nacional Autónoma de México

Keywords:

ERG, Centruroides limpidus limpidus, chemical synthesis, K -channel, scorpion toxin

Abstract

Abstract. A novel toxin named CllErg1 (systematic nomenclature γ-KTx1.5) was purified from the venom of the scorpion Centruroides limpidus limpidus and its amino acid sequence was determined. It has 42 amino-acid residues cross-linked by four disulfide bridges and blocks specifically a potassium channel of the family ether-a-go-go (ERG). The full peptide was chemically synthesized and properly folded, showing that it blocks the human ERG-channels (HERG) with identical affinity to that of the native peptide. Synthetic CllErg1 can be produced in quantities enough to compensate its low concentration in the natural venom. It paves the way to conduct studies aimed at the identification of the structural motifs of HERG critical for proper channel function. Additionally, another analogous peptide CllErg2 (systematic name γ-KTx4.1) was purified and had its full amino acid sequence determined. It contained 43 amino acid residues, maintained closely packed by four disulfide bridge.

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Author Biographies

Fredy I. Corona, Universidad Nacional Autónoma de México

Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology

Cipriano Balderas, Universidad Nacional Autónoma de México

Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology

Liliana Pardo-López, Universidad Nacional Autónoma de México

Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology

Lourival D. Possani, Universidad Nacional Autónoma de México

Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology

Georgina B. Gurrola, Universidad Nacional Autónoma de México

Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology

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2020-08-07

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