A Hydrogen Peroxide Biosensor Based on the Immobilization of the Highly Stable Royal Palm Tree Peroxidase (Roystonea regia) with Chitosan and Glutaraldehyde on Screen-printed Graphene Electrodes

Erika N. Villamizar, Carlos A. Ríos, John J. Castillo


This paper present the fabrication and electrochemical stud-ies of a screen-printed graphene electrode (SPGE) modified with Roy-al palm tree peroxidase (RPTP) in combination with chitosan (CS) and cross-linking of glutaraldehyde (GA) for detection of hydrogen perox-ide. Cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN)6]3-/4-) as a redox species demonstrated an increasing of 50mA evidenced by the electron transfer process of SPGE modified with CS-GA-RPTP. The graphene modified electrode exhibits excellent electrocatalytic activity to the reduction of H2O2, with a linear response in the 100 mM to 5 mM concentration range and a detection limit of 87 mM. The new sensor based on the modification of graphene electrode with the high stable RPTP will provide a potential amperometric detection system for determination of H2O2 in real samples with some biomedical or environmental importance.


Royal palm tree peroxidase; graphene; sensor; hydrogen peroxide.

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Chen, W.; Cai, S.; Ren, Q.; Wen, W.; Zhao, Y. Analyst. 2012, 137,


Lai, G.; Zhang, H.; Han, D. Microchim. Acta. 2009, 165, 159-165.

Li, M., Xu, S.; Tang, M. Electrochim. Acta. 2011, 56, 1144-1149.

Brusova, Z.; Ferapontova, E.; Sakharov, Y.; Magner, E. Electro-analysis. 2005, 17, 460-468.

Gaspar, S.; Catalin, I.; Gazaryan, I.; Bautista, G.; Sakharov, I.; Mattiason, B.; Csoregi, E. Electrochim. Acta. 2000, 46, 255-264.

Alpeeva, I.; Niculescu-nistor, M.; Castillo, J.; Csoregi, E.; Sakharov, I. Biosens. Bioelectron. 2005, 21, 742-748.

Sakharov, I. Biochem. 2004, 69, 1013-1020.

Zamorano, L.; Pina, D.; Arellano, J.; Bursakov, S.; Zhadan, A.; Calvete, J. Biochimie. 2008, 90, 1737-1749.

Walcarius, A.; Minteer, S.; Wang, J.; Lin, Y.; Mercoci, A. J. Mat. Chem. B. 2013, 1, 4878-4908.

Castillo, J.; Ferapontova, E.; Hushpulian, D. J. Electroanal. Chem. 2006, 588, 112-121.

Bin, X.; Chang, G.; Yi, X. Electrochim. Acta. 2012, 62, 327-334.

Niu, X.; Zhao, H.; Chen. C.; Lan, M. Electrochim. Acta. 2012, 65,


Matheus, P.; Abad, M.; Fernández, M. Rev. Tec. Ing. Univ. Zulia. 2007, 30, 225-235.

Sakharov, I.; Vesga, M.; Galaev, I.; Sakharova, I. Plant. Sci. 2001, 161, 853-860.

Shi, L.; Liu, X.; Niu, W. Biosen. Bioelectron. 2009, 24, 1159-1163.

Ahirwal, G.; Mitra, C. Sensors. 2009, 9, 881-894.

Chen, S.; Yuan, R.; Chai, Y. Electrochimica. Acta. 2009, 54, 3039-3046.

Farzana, S.; Ganesh, V.; Berchmans, S. J. Electrochm. Soc. 2013, 160, 573-580

Yagati, A.; Min, J.; Cho, S. J. Electrochem. Soc. 2014, 161, 133-140.

Wan, Q.; Song, H.; Shu, Z.; Wang, J.; Zou, N. (2013) Colloids. Surfaces. B. Biointerfaces. 2013, 104, 181-185.

Zhang, Q.; Qiao, Y.; Zhang, L.; Wu, S.; Zhou, H.; Xu, J.; Song, X. Electroanalysis. 2011, 23, 900-906.

DOI: http://dx.doi.org/10.29356/jmcs.v60i3.95


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Journal of the Mexican Chemical Society (J. Mex. Chem. Soc.) Vol 62, No 2 (2018). Quarterly publication (April-June). Edited and distributed by Sociedad Química de México, A.C. Barranca del Muerto 26, Col. Crédito Constructor, Del. Benito Juárez, C.P. 03940, Mexico City. Phone: +5255 56626837; +5255 56626823 Contact: soquimex@sqm.org.mx http://www.sqm.org.mx Editor-in-Chief: Ignacio González-Martínez. Indexed Journal. Certificate of reserved rights for recurrent publications under digital distribution granted by the Instituto Nacional del Derecho de Autor (INDAUTOR): 04-2018-091118040000-203. Certificate of lawful title and content: Under procedure. ISSN-e granted by the Instituto Nacional del Derecho de Autor (INDAUTOR): 2594-0317. ISSN granted by the Instituto Nacional del Derecho de Autor (INDAUTOR): 1870-249X. Postal registration of printed matter deposited by editors or agents granted by SEPOMEX: IM09-0312 Copyright © Sociedad Química de México, A.C. Total or partial reproduction is prohibited without written permission of the right holder. The Figures/schemes quality and the general contents of this publication are full responsibility of the authors. Updated December 12th, 2018 by Adriana Vázquez (editorial assistant, e-mail: editor.jmcs@gmail.com), J. Mex. Chem. Soc., Sociedad Química de México, A.C.