Behavior of Two and Three Electrode Configuration and Different Mediators in Working Electrode on Development of Disposable Screen-Printing Biosensors for Determination of Free Cholesterol

Authors

  • Mónica Hernández-Cruz Universidad Autónoma del Estado de Hidalgo
  • Carlos Andrés Galán-Vidal Universidad Autónoma del Estado de Hidalgo
  • Giaan Arturo Álvarez-Romero Universidad Autónoma del Estado de Hidalgo
  • María Teresa Ramírez-Silva Universidad Autónoma Metropolitana-Iztapalapa
  • María Elena Páez-Hernández Universidad Autónoma del Estado de Hidalgo
  • José Luis González-Vidal Universidad Autónoma del Estado de Hidalgo

DOI:

https://doi.org/10.29356/jmcs.v57i1.237

Keywords:

Cholesterol, mediator, transducer, amperometric biosensor, screen printing

Abstract

In the present work the development of an amperometric transducer in order to build a free cholesterol biosensor in planar configuration is reported. The one single use disposable biosensors were constructed by screen printing process which is compatible with automated methodologies of production. Based in the incorporation of tetracyanoquinodimethane (TCNQ), Prussian blue (PB) or ferrite (Fe3O4) as mediator or electrocatalytic agent, three types of electrochemical transducers were evaluated. The cholesterol biosensors require a sample volume of 7.2 μL, exhibits good reproducibility and selectivity and cover a lineal answer of 2-16 mM with detection limits from 0.3 to 1.6 mM. The characteristics of biosensors are satisfactory for the decentralized analysis of the lipid in blood since enclose the range of clinical interest (3.5-6.5 mM).

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Author Biographies

Mónica Hernández-Cruz, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

Carlos Andrés Galán-Vidal, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

Giaan Arturo Álvarez-Romero, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

María Teresa Ramírez-Silva, Universidad Autónoma Metropolitana-Iztapalapa

Departamento de Química, Área de Química Analítica

María Elena Páez-Hernández, Universidad Autónoma del Estado de Hidalgo

Área Académica de Química

José Luis González-Vidal, Universidad Autónoma del Estado de Hidalgo

Área Académica de Computación

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Published

2017-10-12

Issue

Vol. 57 No. 1 (2013): Regular Issue

Section

Regular Articles

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