Comparative Study on the N-acylase Activity of Mammalian Kidney Acetone Powders (KAP’s)

Authors

  • Héctor Luna Universidad Autónoma Metropolitana, Unidad Xochimilco
  • Liliana Hernández-Vázquez Universidad Autónoma Metropolitana, Unidad Xochimilco
  • Herminia I. Pérez Universidad Autónoma Metropolitana, Unidad Xochimilco
  • Norberto Manjarrez Universidad Autónoma Metropolitana, Unidad Xochimilco
  • Aida Solís Universidad Autónoma Metropolitana, Unidad Xochimilco
  • Julia Cassani Universidad Autónoma Metropolitana, Unidad Xochimilco

DOI:

https://doi.org/10.29356/jmcs.v57i1.236

Keywords:

Kidney Acetone Poder, Acylase, Enzymatic Resolution, Amino Acids, Deacylation

Abstract

The N-acylase activity and enantioselectivity of mammalian kidney acetone powders (KAP’s) or their enzyme extract was demonstrated on (rac)-N-acetylmethionine; used as reference substrate. It was showed hydrolysis exclusively on the (S)-enantiomer. The biocatalyzed reaction allowed us, to categorize the KAPs regarding the animal source, sheep, pig, calf, bovine, dog and guinea pig as fast biocatalysts reaching equilibrium in around 4 to 5 h; and rat, mouse and hamster were slower biocatalysts, since they did it in around 24 h. In most of the reactions the kidney crude preparations gave a better conversion than the enzyme extract, this fact demonstrated that the longer stirring during the reaction in an aqueous medium, allowed a greater dissolution of the enzyme. These readily available and inexpensive crude biocatalysts have a great potential application in organic synthesis.

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Author Biographies

Héctor Luna, Universidad Autónoma Metropolitana, Unidad Xochimilco

Departamento de Sistemas Biológicos

Liliana Hernández-Vázquez, Universidad Autónoma Metropolitana, Unidad Xochimilco

Departamento de Sistemas Biológicos

Herminia I. Pérez, Universidad Autónoma Metropolitana, Unidad Xochimilco

Departamento de Sistemas Biológicos

Norberto Manjarrez, Universidad Autónoma Metropolitana, Unidad Xochimilco

Departamento de Sistemas Biológicos

Aida Solís, Universidad Autónoma Metropolitana, Unidad Xochimilco

Departamento de Sistemas Biológicos

Julia Cassani, Universidad Autónoma Metropolitana, Unidad Xochimilco

Departamento de Sistemas Biológicos

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Published

2017-10-12

Issue

Section

Regular Articles

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